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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in lots of tumors, which ends up in chromosome instability, and therefore may possibly lead to tumorigenesis. This protein was identified to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a vital function in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been described. Two added splice variants were documented but detailed nucleotide sequence facts just isn't yet readily available.
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Blocking peptides are peptides that bind specifically towards the goal antibody and block antibody binding. These peptide generally is made up of the epitope identified from the antibody.
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This guidebook outlines even further probable optimization of cellular and immunoassay parameters to make certain the very best success are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the concentrate on protein. By evaluating the staining from your blocked antibody versus the antibody on your own, you can see which staining is precise.
Focus on Details Cyclin E belongs towards the extremely conserved cyclin family, whose customers are characterized by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation designs which lead on the temporal coordination of every mitotic party. Cyclin E kinds a posh with and functions for a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S changeover. This here protein accumulates in the G1-S period boundary and is particularly degraded as cells development via S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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